FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50522998
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1360630.2693090382324501No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG644320.12753004087366313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC280600.023.91924
CAGACCG285950.023.5739733
GAACGAT281650.023.29506945
ACCGCGT294350.022.7942166
GACCGCG309850.021.6249075
CCGCGTT310350.021.5972757
CGCGTTC319700.021.000748
TCAGACC332250.020.65472
AGAACGA323250.020.4224544
GCGTTCT339500.019.8090959
TACGGAG338850.019.50188834
ATACGGA340950.019.4938833
AATACGG344600.019.2939332
CTCAGAC416850.018.6703951
AACGCTA197150.018.37416523
ACGCTAC197500.018.36438624
CCCAATA371750.018.07851429
ACGGAGA370200.017.89293335
TCGGCGC131200.017.71547145
ACGTTCG33900.017.6548066
Produced by FastQC (version 0.11.5)