FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75613315
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC210500.016.1855775
ACGGACA208600.016.13601118
TCACCCG215800.015.985043
CGGACAC214050.015.74250313
CCGGACA232400.014.64532612
AGCTTAG221250.014.32952245
CGGACAG232750.014.24896319
CACCCGG241550.014.2439854
GGCATAG235500.013.52143835
ACACGGA250950.013.38336816
CACGGAC252350.013.3555517
GCCCGGA263350.013.30145310
ATAGCTC251650.012.94900639
GACACGG260950.012.85287115
CGGCCCG280050.012.4849938
GAGCTTA262900.012.31681444
CATAGGG269000.011.96293937
GCATAGG278400.011.55909436
GTCACTC362700.011.53200158
TAGCTCT283850.011.52821240
Produced by FastQC (version 0.11.5)