FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51773259
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT681300.1315930295212824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT147450.025.5407146
AGACCGC149700.025.2470044
GACCGCG151750.024.816995
CCGCGTT159000.023.68547
CAGACCG161250.023.6199843
CGCGTTC163900.023.1282798
GAACGAT149800.022.08896645
AATACGG188750.019.71719632
ATACGGA189200.019.6097233
TACGGAG191750.019.30317734
TCAGACC202600.019.2551982
CAATACG203750.018.32083931
AGAACGA183100.018.2154744
GCGTTCT213050.017.8454519
AAGAACG193900.017.25892343
ACGGAGA224600.016.57991835
CTCAGAC285000.016.2328031
AACGCTA120250.016.14548323
ACGCTAC122000.015.93286624
CCCAATA245850.015.32971529
Produced by FastQC (version 0.11.5)