FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51773259
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1007490.19459659667165244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT433800.016.8210663
GGTCACT432350.016.258287
AGCTTAG305250.016.09939245
GTTGTCG32150.016.02731526
GGAGGTC452150.015.9827884
GTCACTC445000.015.9579018
AGGTCAC451100.015.9188846
GGCATAG310100.015.915853535
ATAGGGG316650.015.75510638
GAGGTCA466800.015.5576295
ACTCTCC477850.014.86671111
TGGCATA337500.014.71156634
GAGCTTA342450.014.46907244
TGGGAGG369750.014.2000632
TCACTCT540100.013.3211569
CACTCTC539550.013.17137810
CATAGGG378000.013.16236437
ATATAGC47800.012.8096076
CTGGGAG458900.012.775011
GCATAGG404050.012.30364636
Produced by FastQC (version 0.11.5)