FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43657388
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT39000.017.841941
TTCGACG28000.017.6765636
CCCGTAT35650.016.802361
CTCCGTT75950.016.366641
CTCGGTT94150.016.312191
CGACGAG29900.015.8011518
TCGACGA30800.015.5584937
CGGTATA41900.015.35704320
CTCCGGT61800.015.2312671
GTCCGAT26450.015.1545321
CCCGGTT41600.014.8863271
CCCGGAT99900.014.8097631
AGACGCT149950.014.7483641
CCCCGTT58450.014.7172641
GCACTAC60100.014.60350525
AGGGATA112350.014.5777844
CCCCGCA143500.014.51816435
AACGCTA89100.014.36930823
TAATTAT120750.014.3092129
AAGCGGC147900.014.25789328
Produced by FastQC (version 0.11.5)