FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41166686
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG109850.013.24919935
AGCTTAG114650.013.04752745
ATAGGGG117050.012.95296438
CATAGGG115600.012.57061137
GCATAGG121150.012.08757736
ACCCGGC54550.011.881655
GAGCTTA132150.011.28566144
GGGAGGT175200.011.200673
TGGCATA135000.011.0476934
GTCACTC166100.010.9481128
GGAGGTC170200.010.7233194
GGTCACT167500.010.60127457
CGTAACG10800.010.41422442
ACTCTCC180250.010.36335411
CACCGAA178800.010.11507745
CACCCGG67900.09.976194
TAGGGGA177550.09.8822139
GCACCGA182200.09.7040944
ACGGACA71800.09.55906418
CGGACAC69900.09.43378213
Produced by FastQC (version 0.11.5)