FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61034085
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG205550.014.079761545
ATAGGGG221250.013.03928538
GGCATAG223450.012.75877435
GGGAGGT347550.012.4111243
GGTCACT328300.012.1879577
GAGCTTA240100.012.06924644
GGAGGTC354450.011.7635534
GTCACTC348300.011.63668258
GAGGTCA365800.011.6090535
TGGCATA253900.011.4679134
AGGTCAC367100.011.3779116
CATAGGG259150.011.06285537
ACTCTCC367800.011.0314611
GCATAGG277300.010.33001236
TCACTCT405100.010.166189
CACTCTC400100.09.96699110
GTTGTCG34900.09.9269526
TAGGGGA435300.09.16060339
TAATCGG53050.09.07708554
AGGGGAG447800.09.03052540
Produced by FastQC (version 0.11.5)