FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55159763
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT641150.11623508969753912No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG562540.10198375942985832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT124500.025.784526
GACCGCG129000.024.8690665
AGACCGC131250.024.4092754
CCGCGTT133100.024.08477
CGCGTTC139450.022.9879788
CAGACCG147750.021.7747293
TTCGACG30000.021.2961716
ACGCTAC166600.017.84228124
TCAGACC185700.017.8336432
AACGCTA166850.017.81499723
TCGACGA35300.017.780097
GAACGAT183400.017.59862345
AATACGG186500.017.14501232
ATACGGA187550.017.00115833
CGCTACC175550.016.88145425
TACGGAG189050.016.87738634
GCGTTCT190800.016.8719679
CGGTATA48450.016.7637920
TCGGCGC79450.016.40263445
CGACGAG39350.015.9501198
Produced by FastQC (version 0.11.5)