FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55159763
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG166500.014.08298335
AGCTTAG167700.013.98001545
ATAGGGG175000.013.86155838
CATAGGG185300.012.81201637
GTCACTC252400.012.44898
GAGCTTA194450.012.19583544
GGTCACT258250.012.1407747
GGGAGGT287150.012.1083983
GGAGGTC272750.012.0720144
GTTGTCG34500.012.00359526
AGGTCAC272800.011.9720216
GCATAGG200850.011.8088536
TGGCATA207650.011.519464534
GAGGTCA294450.011.2215965
ACTCTCC291900.011.02654211
TAGGGGA296900.010.57197939
GCGTCGA26800.010.40688211
CGTCGAT26550.010.250874512
AGGGATC132600.010.1087682
TGTCGTT42550.09.89138328
Produced by FastQC (version 0.11.5)