FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53108520
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG603410.11361830455828933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA164750.023.4055323
ACGCTAC167100.023.03597624
CGCTACC173450.022.205625
AAAACGC190400.020.37052321
TCACGCT207150.019.4402588
AAACGCT206000.018.89342922
GAAAACG205550.018.8253420
TTCACGC216700.018.7939727
TTCGACG22150.018.3867176
ACGCTCA221400.018.1584710
CCGGCTT209050.017.3814132
CACGCTC243600.016.5128699
ACCGCGT60500.016.3642716
CGTGGTT243550.016.10108241
GCTACCT243400.016.01810326
GTTTTCG247250.015.73481545
GCCGGCT211950.015.7255451
GTCCGTT39700.015.7132721
TCGTGGT249300.015.62142140
GACCGCG63250.015.5896755
Produced by FastQC (version 0.11.5)