FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53226955
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1043170.19598528602660062No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCT829650.15587027287208144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG160500.033.471146
CGACGAG168800.031.4153738
TCGACGA171200.031.3531237
GTTCGAC172500.031.2724535
CGGTATA259400.028.97832120
AGTTCGA188050.028.864544
CAGTTCG197500.027.7094423
CGAGGAT233300.026.78622611
TATAGAG281100.026.51695423
ATAGAGT281950.026.44536224
GACGAGG257650.026.2384019
ACGAGGA260900.026.15963410
TAGAGTT298900.025.00869425
ATGACGG279600.024.97679116
GGTATAG281600.024.5308121
GTATAGA307900.023.96427522
TGACGGT297900.023.412517
GATGACG310050.022.23588615
ACTCATT502200.021.67870535
GCCGGCT376000.021.509951
Produced by FastQC (version 0.11.5)