FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53226955
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1332660.25037314270560845No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA911320.17121400237905776No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG724110.13604197347002095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA261400.023.44349945
CATGCAT181350.020.2887349
ATACATG211200.017.924886
TGCATTC210300.017.66629211
TACATGC210600.017.472847
CGGTATA24600.016.82729320
AAACTCT390300.016.01249744
AAAACTC404500.015.595143
TATATAC138500.015.4375367
ACATGCA243050.015.3985838
TTCCATA154200.014.8627992
GCATTCC261700.014.47065512
TCCATAT159000.014.2300413
ATATATA135100.014.0774986
ATATACA166000.013.9505548
CATACAT113350.013.9031145
TCATACA116800.013.64674
CCATATA132050.013.5710014
GACGGTA30200.013.55863518
ATTCCTT509700.013.40178814
Produced by FastQC (version 0.11.5)