FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51874225
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT122700.020.2219476
AGACCGC123050.020.182964
GACCGCG125650.019.8547865
GAACGAT125650.019.70374145
CAGACCG133850.019.0588323
CCGCGTT133500.018.5860127
CGCGTTC144500.017.046628
AGAACGA150950.016.38807344
ATACGGA153600.016.2482333
TACGGAG152950.016.23139434
AATACGG156450.015.80740732
CAATACG161250.015.3068431
ACGCTAC76750.015.24172424
AAGAACG162850.015.21767143
AACGCTA77400.015.05531923
TCAGACC180850.014.3794052
GCGTTCT179050.013.945759
CGCTACC80650.013.8073425
ACGGAGA183950.013.66748635
CCCGTAT30550.013.6323371
Produced by FastQC (version 0.11.5)