FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51874225
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG91400.010.90549638
AGCTTAG100850.09.93656545
GGCATAG100250.09.87578735
GGGAGGT181550.08.8473063
CATAGGG116350.08.56666637
GTCACTC172000.08.4613678
GCATAGG119450.08.45768336
TCAGACG83300.08.3988842
TAATCGG25600.08.1712254
GAGCTTA126750.08.05981544
GGAGGTC194700.07.94816064
TGGCATA130850.07.859097534
GGTCACT180550.07.7860327
TTAACGG65550.07.75533258
CAGACGT92550.07.4865793
CGGCTAA42950.07.33485131
ACTCTCC209100.07.294678711
ATCGGTT37100.07.2751556
AGGTCAC218450.07.21773436
GTTGTCG24150.07.174042726
Produced by FastQC (version 0.11.5)