FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48979949
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1909800.3899146567098304No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG709280.14481027736472327No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG508930.1039057839770311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT347050.028.28448145
AACGCTA185100.023.9293923
AGACCGC409250.023.8060024
ACGCTAC186350.023.79301824
AGAACGA413900.023.78704344
ACCGCGT410600.023.5578966
GACCGCG409650.023.5521245
CAGACCG420900.023.2320963
CCGCGTT417000.023.1908957
CGCGTTC419450.023.0554358
CGCTACC196350.022.58238225
AATACGG447350.021.98521632
ATACGGA448450.021.8710833
TCAGACC462400.021.7940462
AAAACGC204700.021.78072521
TTCGACG26900.021.7424286
TACGGAG450300.021.69058234
AAGAACG463750.021.17120243
CAATACG467750.021.13000531
GAAAACG216450.020.6087120
Produced by FastQC (version 0.11.5)