FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52818410
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1808100.34232382231877106No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1271130.24066040609704076No Hit
GGGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGAC720090.13633314596179627No Hit
CCCTCGTCCAACCAGCTGCCACGTCCTGTAACCAAAAGTGACCGGGATGAA716610.13567428478062857No Hit
GGACGGGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAA706160.13369580795786926No Hit
GTGCAAATTAAATTCAGAAGGGACGGGGGAAACAGAGTCGTGGAGGCTTTG681360.12900047540242124No Hit
GGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACA677630.12829428223984782No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG653920.12380531712332878No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG628660.11902289372209425No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA589690.11164478446056972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG59250.028.3665396
TCGACGA64450.026.706037
GTTCGAC65150.026.5228945
CGGTATA92750.025.34972220
CGACGAG65700.025.20488
AGTTCGA78500.022.270294
ATAGAGT132700.019.27796424
ATGACGG109150.019.04695716
GTATAGA132700.018.63363522
GACGGTA125750.018.32171418
CGAGGAT111350.017.66022111
GTTCCTA128950.017.50079515
CCGGCTT266800.017.3975432
ACGAGGA127500.017.31133310
GAACGAT353250.017.23535245
GCCGGCT227300.016.962111
GACGAGG131700.016.6225919
ACGGTAT143400.016.53722819
GGTATAG144450.016.52603521
AACGCTA241700.016.29041923
Produced by FastQC (version 0.11.5)