FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47318086
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG143150.013.79105638
GGCATAG142850.013.4858735
AGCTTAG147200.013.26796245
GTCACTC196650.012.9157818
CATAGGG155750.012.44412537
GAGCTTA161500.012.16303244
GCATAGG161050.012.033977536
AGGTCAC216850.011.9928346
GGGAGGT231300.011.9738893
GGTCACT210150.011.8614167
GGAGGTC222500.011.7395474
GTTGTCG29250.011.6143226
TGGCATA174400.011.26260734
GAGGTCA241800.010.9695955
ACTCTCC234800.010.874660511
AACTCTA111000.010.19617545
TCACTCT268800.09.7585949
CACTCTC263900.09.70109610
TAGGGGA258750.09.46537439
TGTCGTT36300.09.17273128
Produced by FastQC (version 0.11.5)