FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50928932
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT845560.16602743603576842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT160300.026.4262686
GACCGCG164500.025.833615
AGACCGC164900.025.7300154
CCGCGTT169850.024.9404227
GAACGAT168150.024.80912245
CGCGTTC178700.023.730448
CAGACCG181850.023.492763
AGAACGA195900.021.41477244
AATACGG199100.021.09973132
ATACGGA202300.020.74352533
TACGGAG202700.020.70200234
TCAGACC213500.020.4953062
GCGTTCT226000.018.8733469
CAATACG227350.018.53761931
AAGAACG228600.018.40076643
ACGGAGA234100.017.98291235
CTCAGAC278450.017.685871
CCCAATA249650.017.15069229
CGGAGAG256650.016.35053836
CCAATAC269650.016.14597930
Produced by FastQC (version 0.11.5)