FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43016948
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT36300.019.1048161
AACGCTA93550.015.873960523
ACGCTAC93550.015.8270924
CGCTACC97350.015.25665225
CCCGTAT30550.015.2566051
CTCGGTT99550.015.13155651
CCCGGAT101450.015.0035291
GTCCGGT33050.014.7838331
GTCGGTT39100.014.5118431
AGACGCT159200.014.470160541
CTCCGGT58950.014.2470121
CTCCGAT43850.014.1722321
CTCCGTT68000.014.0727441
ACGGTAC58200.013.87702945
CTACACG43050.013.84834245
AAGCGGC160250.013.70899228
CCCCGCA159400.013.66294835
CCGTACG45000.013.6478914
AAAACGC111000.013.58027921
GTCCGAT25000.013.5098331
Produced by FastQC (version 0.11.5)