FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51329568
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG516360.10059698924409417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174900.014.9559645
GGCATAG179550.014.63933635
ATAGGGG184850.014.44994938
GTCACTC258950.013.6955678
GGTCACT269850.013.083947
CATAGGG205500.012.91110237
GGGAGGT303550.012.8892713
GAGCTTA207500.012.63851444
GGAGGTC291400.012.5403034
ACTCTCC287750.012.448194511
TGGCATA214600.012.40592434
GCATAGG216400.012.26059536
AGGTCAC312100.011.8441246
GAGGTCA334750.011.2009595
GTTGTCG26150.011.18621126
CACTCTC329100.010.76953810
TCACTCT337750.010.7521189
TAGGGGA329300.010.57176939
CGTACGC16300.010.3431645
ACCCGGC82600.010.0693495
Produced by FastQC (version 0.11.5)