FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52123404
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT939900.18032206799080122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT220600.022.4087286
AGACCGC224350.022.325234
GACCGCG220600.022.3069445
TTCGACG28550.022.3034976
AACGCTA161750.021.61680223
CCGCGTT231500.021.343917
CAGACCG237600.021.335933
ACGCTAC163650.021.31089624
GAACGAT243900.021.21731245
CGCGTTC233450.021.213438
CGCTACC174750.019.93116225
AAAACGC185300.019.00304621
TCAGACC285950.018.6423972
CGGTATA45600.018.30601320
AATACGG284050.017.84609432
GAAAACG194800.017.81066520
AGAACGA291800.017.78074644
GCGTTCT282400.017.671399
TCACGCT207650.017.5970088
TCGACGA36250.017.5038557
Produced by FastQC (version 0.11.5)