FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52123404
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC106600.012.6686155
AGCTTAG167350.012.15380745
GGCATAG172200.011.8623735
CGGACAC114400.011.841989513
CCGGACA119050.011.62500912
CGGACAG118000.011.38213119
TCACCCG128500.011.1753153
ATAGCTC119850.010.96338939
ACGGACA123700.010.89387918
GGGAGGT308150.010.861533
ACACGGA126450.010.83570616
GTTGTCG33700.010.68041126
CACCCGG131750.010.6604864
GAGCTTA201450.010.24211344
ATAGGGG210850.010.23275338
TCAGACG112850.010.1923632
GACACGG133600.010.17276115
TGGCATA204150.010.1496434
GTCACTC273950.010.0793978
GGAGGTC285650.010.0702364
Produced by FastQC (version 0.11.5)