FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51692718
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA2366890.45787687155471296No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG2004050.3876851667966076No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1823910.3528369314997134No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG980500.18967855395028754No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA908030.17565917118151922No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC728190.1408689711382559No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT724490.14015320301014159No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA687300.13295876606836576No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG685930.13269373841011803No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT678020.13116354222271695No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA658800.12744541697343134No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN651350.12600420817493094No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA643040.12439663164935534No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA637510.12332684847409263No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG627640.12141748862963639No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC607800.11757942385618028No Hit
CACCCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCA534060.10331435851370786No Hit
TTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTG526050.10176481724176314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGG153050.018.7791671
ACCCGGG157500.018.03342
GGGAGGT737350.017.8813953
TGGGAGG556450.017.7214092
CTGGGAG580000.017.634831
GGAGGTC747000.017.46494
GTTGTCG59450.017.10849426
GAGGTCA764850.017.1065435
AGGTCAC786200.016.509776
GGTCACT812600.015.8685567
GTCACTC823650.015.6671678
AGCTTAG616800.015.66678645
ACTCTCC847100.015.15214311
TCACTCT868300.014.8617979
CACTCTC866950.014.75826510
CTCCCCA672850.014.30149114
GGCATAG672300.014.26545635
ATAGGGG677300.014.25584438
TGGCATA688250.013.99143134
TGTCGTT74850.013.91978528
Produced by FastQC (version 0.11.5)