FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45947896
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1017200.22138119229659614No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT484820.10551516874679093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT270000.020.678388
AACGCTA272250.020.03329523
ACGCTAC273550.019.93090824
TTCACGC289200.019.39117
CGCTACC279850.019.37769325
CCGGCTT309000.019.342122
ACGCTCA301750.019.09898610
GCCGGCT294800.018.9352071
AAAACGC294350.018.56412921
CGGCTTC342100.018.2534263
CACGCTC312300.017.9279829
GAAAACG303850.017.92372320
AAACGCT314650.017.42671222
GTTTTCG322500.016.70448345
CGTGGTT326300.016.66130841
CCCGTAT31600.016.460611
TCGTGGT332600.016.37612240
CTACCTC342850.016.04671127
GTTAGTT147250.016.00167844
TTCGACG17000.015.4816696
Produced by FastQC (version 0.11.5)