FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45947896
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG723800.15752625539154178No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA534910.11641664723886379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG207400.017.51921745
GGCATAG238900.015.2149635
GGGAGGT329850.015.0607513
ATAGGGG249450.014.74413838
GAGCTTA253250.014.53279644
GGAGGTC336250.014.4139784
TGGCATA266750.013.69847134
GTCACTC344050.013.6820748
GGTCACT348550.013.4989747
ACTCTCC350200.013.345242511
CATAGGG280150.013.05610337
AGGTCAC386200.012.6024416
GCATAGG292600.012.52294136
GAGGTCA394450.012.4187885
TGGGAGG314000.012.2808972
TCACTCT391100.012.14541159
CACTCTC398400.011.74778110
CACCGAA236000.011.5804845
TCAGACG88600.011.4335532
GCACCGA238600.011.13511844
Produced by FastQC (version 0.11.5)