FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56861074
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC109200.014.2233875
ACGGACA116700.013.10910818
ATAGCTC113250.013.01193739
AGCTTAG156200.012.51578645
CGGACAC127050.012.43218813
TCACCCG135550.012.3904053
CGGACAG127000.012.01049819
ACACGGA129050.011.95921516
GGCATAG167700.011.60498135
CCGGACA145350.011.42386412
TAGCTCT130950.011.32140840
GACACGG138550.011.107285515
GAGCTTA182700.010.77399344
TGATAGC142800.010.76077637
GTTGTCG32350.010.57037126
TCAGACG134450.010.5662862
CACCCGG155100.010.5077444
CACGGAC149050.010.50560617
ATAGGGG191150.010.32176138
CATAGGG194650.010.32162737
Produced by FastQC (version 0.11.5)