FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42060163
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29050.025.2467046
TCGACGA33550.022.0619477
CGGTATA47900.020.38492620
CGACGAG35800.020.3611568
GTCCGTT36050.016.9286541
GTTCGAC45550.015.9037945
ATGCGCG18850.014.7994744
CCCGTAT26450.014.4737671
CTCGGTT90000.014.2122941
AACGCTA115400.014.19482623
AGACGCT190900.014.1420141
TGCGCGT19650.014.08245545
ACGCTAC116700.013.94135624
ACGAGGA68200.013.92097310
ACCGCGT31600.013.8829186
CTCCGTT62500.013.8359691
ATGACGG65550.013.763329516
AAGCGGC181750.013.55884528
CTCCGGT59250.013.5306921
CCCCGCA185950.013.50251535
Produced by FastQC (version 0.11.5)