FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50027863
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA941460.1881871308394684No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG514970.10293663752937038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG50600.027.1989526
TCGTATG55300.027.0077445
CGTCCCG55600.026.3301335
ACCCGTC56800.025.38101232
TCGACGA56800.023.8748877
CGACGAG56000.023.095228
GTTCGAC57600.023.0763195
CGGTATA82050.020.62794920
GCCGGCT199350.020.6194341
CGTCTGA73800.020.13694816
GTCCCGA73100.019.9950236
CCGGCTT237150.018.9141162
ACGTCTG80250.018.462615
TTCACGC239100.017.8961937
CAGTTCG80250.017.8490413
TCGTTAC42400.017.76401544
TCACGCT242100.017.6373988
CACCCGT83200.017.48912631
ACGCTAC241250.017.39698424
AACGCTA242600.017.33696723
Produced by FastQC (version 0.11.5)