FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50027863
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260930.2520455450995378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC48300.023.07538443
GTCGCCG48400.023.02532444
GCGTCGT53600.020.04644611
TCGTGTA59550.018.0788914
CGTCGTG61400.017.49982812
GTCGTGT63250.017.19859513
TGGTCGC67550.016.8291942
AGCGTCG73600.015.44536410
TCTCGGT71300.015.32024436
TCGCCGT60050.014.63236145
ATCTCGG86950.013.91883935
GTAGATC85150.013.53179631
CTCGGTG85900.013.39017737
TAATCGG116650.012.7968934
AACTCTA126700.012.68610345
TCGGTGG94450.012.55492638
CGGTGGT89300.012.53156939
TGTAGGG92300.012.43528517
ATAATCG125150.012.2478523
GTAACGA120900.012.08778818
Produced by FastQC (version 0.11.5)