FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47594003
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG722080.15171659336996723No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT597470.1255347233557976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT140450.022.424436
AGACCGC141500.022.10624
CCGCGTT150600.020.8978677
CGCGTTC152900.020.5394368
GACCGCG152900.020.5315195
CAGACCG156400.019.942623
AACGCTA214750.018.55370523
ACGCTAC217000.018.34190224
GAACGAT174350.017.88294645
CGCTACC226000.017.5816825
GCCGGCT225150.017.5481991
TCAGACC186950.017.0933172
AAAACGC235600.017.04423921
TCACGCT247550.016.9301388
ACGTTCG14500.016.911176
TTCACGC251050.016.855357
ACGCTCA256900.016.67221810
GCGTTCT191450.016.4968979
CCGGCTT257800.016.4111272
CACGCTC254900.016.4059019
Produced by FastQC (version 0.11.5)