FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46896586
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG590140.1258385844973875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG34750.016.63839126
ACCCGGC113750.015.4532325
ACGGACA114150.015.33422218
CGGACAC116700.014.96149113
TCACCCG120600.014.7637323
CGGACAG123350.014.02665219
AGCTTAG224550.013.96683745
GGCATAG227750.013.71125235
TGTCGTT44150.013.70735628
CCGGACA127900.013.59882112
ATATAGC49150.013.5066836
GGAGGTC342750.013.0876064
CACCCGG140700.013.0534834
GGGAGGT386550.012.9799013
GTCACTC342600.012.8336558
CACGGAC141050.012.53734817
ATAGCTC132300.012.44746839
ACTCTCC357400.012.31456711
ACACGGA142100.012.28598516
TGGCATA255650.012.28541434
Produced by FastQC (version 0.11.5)