FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51964138
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG916690.17640819905450947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG282300.016.42060545
GGGAGGT404600.015.6469423
GGTCACT399350.015.2869817
GGAGGTC406650.015.2134824
GTCACTC408350.014.9986768
GGCATAG313200.014.8034535
AGGTCAC424300.014.7803276
GTTGTCG25900.014.77822126
ATAGGGG323100.014.4976738
GAGGTCA437050.014.3424895
ACTCTCC435950.014.17319111
GAGCTTA330300.014.0844544
TGGCATA339050.013.8009434
TGGGAGG366700.013.2280222
TCACTCT483850.012.8440599
CATAGGG378950.012.3390537
CACTCTC501250.012.22747710
GCATAGG401050.011.69364636
CTGGGAG484500.011.2661751
TCTCCCC570150.010.84160613
Produced by FastQC (version 0.11.5)