FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47799531
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT639160.13371679316267768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC134950.024.7585984
ACCGCGT138650.024.0769216
GACCGCG143400.023.315375
CCGCGTT147400.022.662927
CAGACCG148600.022.5906313
GAACGAT145050.022.49431845
CGCGTTC150750.022.1742218
AATACGG160500.020.47860132
ATACGGA162050.020.19862233
TACGGAG162050.020.05980134
AGAACGA166200.019.73507944
AACGCTA102800.019.2575123
TGCGCGT62400.019.05672845
ACGCTAC104600.018.96982424
TCAGACC183900.018.6708682
TTCGGCG119300.018.34783244
ATGCGCG64950.018.21876544
GCGTTCT186200.018.0008729
CGCTACC113400.017.47801225
ACGGAGA188100.017.38935335
Produced by FastQC (version 0.11.5)