FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51458508
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT538160.10458134542105262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT105350.024.9832386
GACCGCG107100.024.5125275
CCGCGTT112100.023.4186947
AGACCGC111950.023.3905854
CGCGTTC113350.023.1604378
CAGACCG123050.021.5002213
AACGCTA75450.021.4709123
ACGCTAC76650.021.19320924
GAACGAT129900.020.39594345
AATACGG130600.020.13984532
ATACGGA134300.019.60383633
TACGGAG135700.019.53401834
CGCTACC83300.019.39372625
AGAACGA146600.018.11859544
TCAGACC158450.017.3642862
AAAACGC94200.017.36227621
TTCGGCG71400.017.02440644
CAATACG159350.016.76072331
AAGAACG158350.016.7598343
ATGCGCG36400.016.75885644
Produced by FastQC (version 0.11.5)