FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48427116
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT590760.12198950687048968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21300.020.0697696
GACCGCG161500.018.4034235
AGACCGC164650.018.2973084
ACCGCGT164350.018.139056
GAACGAT171050.017.87576945
CAGACCG178050.017.1729853
CCGCGTT176350.016.8792327
CGCGTTC177500.016.8079018
ACGCTAC118450.015.61370624
AACGCTA119300.015.55900623
CGGTATA37800.015.41616520
CGCTACC122250.015.12837325
AGAACGA205500.014.96666544
TCGACGA28900.014.9476097
ATACGGA210450.014.46497333
TACGGAG208900.014.45382834
GTCCGTT41200.014.437861
AATACGG211550.014.38976132
TCAGACC222250.014.3752352
CAATACG226800.013.50155731
Produced by FastQC (version 0.11.5)