FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48427116
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG121500.013.01785945
ATAGGGG123850.012.75579438
GGCATAG125350.012.35105235
CATAGGG135000.011.5188937
GAGCTTA142700.011.13143344
GCATAGG142800.010.85761636
TGGCATA155600.010.29601234
GGGAGGT220300.010.223783
AGGTCAC223650.09.8074456
GTTGTCG26200.09.70353626
GTCACTC212700.09.6464738
GGAGGTC226000.09.5371874
ACTCTCC221000.09.40672411
GAGGTCA244100.09.2257415
GGTCACT223850.09.0451347
TAGGGGA228900.08.868093539
TCAGACG63800.08.5000312
GCGTAGG59350.08.37994339
GGCGTAG60800.08.21704738
CGTCGAT17350.08.03990612
Produced by FastQC (version 0.11.5)