FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49612963
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1973600.3977992606488752No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG553910.11164622439502353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC359700.026.542914
GACCGCG361150.026.3678025
ACCGCGT361700.026.2837626
CCGCGTT378600.025.1080767
CAGACCG385350.024.7820513
GAACGAT383250.024.61666945
CGCGTTC394750.024.1150518
TCAGACC412500.023.4211852
AATACGG412200.022.9092132
AGAACGA417850.022.65367744
TACGGAG417750.022.51869834
ATACGGA420850.022.4864633
CAATACG437200.021.6711131
GCGTTCT444300.021.5573149
CTCAGAC488550.021.2493861
ACGGAGA449250.020.98483335
AAGAACG451700.020.9610143
CGGAGAG466050.020.1315436
CCCAATA477400.019.98306729
CCCCAAT485250.019.60843528
Produced by FastQC (version 0.11.5)