FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70843023
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT864650.12205153921791283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT183100.025.8914076
GACCGCG183150.025.7861145
AGACCGC188250.024.9919284
TTCGACG39450.024.5245426
CCGCGTT198050.023.902727
CAGACCG205100.022.972163
CGCGTTC209350.022.7404568
GAACGAT210400.021.4725245
AATACGG233050.020.36033232
ATACGGA234000.020.26805933
CGGTATA63800.019.36008320
TACGGAG246000.019.32510234
TCAGACC253850.019.0761832
CAATACG251400.019.05319431
AACGCTA180150.018.18369123
AGAACGA253750.017.93736344
ACGCTAC184400.017.80120324
AAGAACG255350.017.75437743
TCGACGA55750.017.2329437
GCGTTCT278450.017.2264529
Produced by FastQC (version 0.11.5)