FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54715052
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1054840.19278790048486108No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG609760.11144282564147065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT238150.022.49027445
ACCGCGT247200.021.5210026
GACCGCG247750.021.4459885
AGACCGC251550.021.3724064
TTCGACG27500.021.3494956
AACGCTA170150.021.02126723
ACGCTAC172250.020.72582224
CCGCGTT257350.020.6547037
CGCGTTC261600.020.3105818
CGCTACC180550.019.81201425
CAGACCG273950.019.7720763
AAAACGC194450.018.63675121
AGAACGA300700.017.87556544
TCAGACC313500.017.8373642
AAACGCT205300.017.58606522
AATACGG307800.017.486932
ATACGGA313200.017.17105333
TCACGCT217500.017.1373428
GAAAACG210200.017.11184120
GCGTTCT312950.017.0857669
Produced by FastQC (version 0.11.5)