FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48403683
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT534260.1103758984621067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC109900.023.6053814
GACCGCG111050.023.4014535
ACCGCGT111550.023.2503596
CAGACCG125150.020.998653
CCGCGTT124400.020.8482637
CGCGTTC124650.020.7523338
AACGCTA141150.020.19219823
ACGCTAC141800.020.13375924
TTCGACG18500.020.0623666
CGCTACC148100.019.18826725
GAACGAT142350.018.0771345
AAAACGC161350.017.85841421
AATACGG150400.017.14081832
TCAGACC158600.016.8962122
TCACGCT178750.016.8120948
ATACGGA156200.016.59057433
TACGGAG155650.016.5334834
GAAAACG177050.016.3256320
AAACGCT177800.016.15595422
GCGTTCT168550.015.574129
Produced by FastQC (version 0.11.5)