FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46767048
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1496920.32008007005274314No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1186130.2536251593215804No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT476580.10190508496495225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT298100.025.76004445
GCCGGCT349250.025.0718081
ACCGCGT318750.024.5737386
AGACCGC321850.024.3929794
TCACGCT379150.024.288638
GACCGCG324000.024.1269365
CCGCGTT326900.023.9517157
CCGGCTT397800.023.6783712
CGCGTTC330200.023.6578338
CAGACCG333500.023.6488253
ACGCTCA396200.023.41944110
GAAAACG279500.023.29641220
TTCACGC399600.023.0963047
TTCGACG24850.022.7281826
TCAGACC358450.022.26642
CACGCTC417050.022.1676859
AGAACGA348900.022.11255344
CGGCTTC444850.021.629253
GCGTTCT378900.020.6824119
AACGCTA349600.020.31130823
Produced by FastQC (version 0.11.5)