FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46767048
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1194750.25546833744990705No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG674300.1441827160012323No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA641410.1371499864605523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT476350.017.2124483
GGAGGTC478500.016.956474
GTTGTCG35700.016.82716426
AGCTTAG368950.016.19160345
TGGGAGG393350.015.8061452
GGCATAG391800.015.11778735
ACTCTCC532700.014.93297611
GGTCACT533550.014.9118867
ATAGGGG407350.014.801023538
GTCACTC541350.014.7718258
ATATAGC46450.014.7217416
GAGCTTA411900.014.57429944
TGGCATA412950.014.42768834
CTGGGAG476900.014.0945591
TGTCGTT44550.013.98971128
CACCCGG130650.013.8606851
TATAGCT51600.013.4704297
CACTCTC601050.013.33493310
AGGTCAC615000.013.2587996
AGGCTCT465600.013.14453320
Produced by FastQC (version 0.11.5)