FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53355523
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG634390.11889865647085869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28600.020.5311376
ACCGCGT63600.018.1113726
AACGCTA196000.017.70157823
ACGCTAC197950.017.55008124
AGACCGC66950.017.0741044
CGCTACC204750.016.95639225
TCACGCT215900.016.9227878
GACCGCG70100.016.4349885
TCGACGA35600.016.3677237
AAAACGC215850.016.20827521
GTCCGTT43800.016.1616551
ACGCTCA245150.015.66535110
CTACACG68000.015.18597645
TTCACGC244750.015.1210417
GCCGGCT252950.015.061991
CCGGCTT271750.015.0378222
CGACGAG38400.014.9398918
TACGGTA82900.014.89898644
GAAAACG234350.014.89030720
ACGGTAC83650.014.84608845
Produced by FastQC (version 0.11.5)