FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43957532
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20500.022.0604576
TCGACGA27200.017.8661697
AGACCGC110100.017.6359844
ACCGCGT108550.017.5560116
GACCGCG113600.016.9341745
GTCCGTT29600.016.8790761
CGGTATA34650.016.03801720
CCGCGTT122050.015.6869457
CAGACCG126050.015.6542753
GCGGACT62900.015.5620292
GTTCGAC30850.015.0240945
CTACACG79900.014.86644245
GTTCCTA68300.014.82272415
AACGCTA217600.014.64268223
ACGCTAC217800.014.6193224
CGCGTTT35900.014.4127858
CCCGTAT24700.014.3961891
GAAAACG175800.014.15445720
GCGTATG66750.013.99082238
CGCTACT110150.013.93248225
Produced by FastQC (version 0.11.5)