FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51575612
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG144200.013.18867845
ATAGGGG153100.012.54926238
GGCATAG154300.012.29098235
GTTGTCG35300.011.918076526
GAGCTTA171300.011.29779244
GGGAGGT245400.011.1951323
GGAGGTC248100.010.6927414
GTCACTC248850.010.3730328
AGGTCAC259800.010.3159586
GCATAGG182550.010.30267136
CATAGGG184150.010.27431837
GGTCACT249400.010.11531457
ATATAGC51600.010.0739366
GAGGTCA276800.09.99105555
TGGCATA192050.09.98045334
ACTCTCC267350.09.84043411
TCAGACG78850.09.530242
TATAGCT56650.08.8985317
GTCGTTC51900.08.84276729
TCACTCT302700.08.6763769
Produced by FastQC (version 0.11.5)