FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46845685
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT547240.11681758949623643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT114600.025.4637746
GACCGCG116650.025.074145
AGACCGC123500.023.7198244
CCGCGTT125800.023.160967
CGCGTTC130050.022.507878
CAGACCG132500.022.3124313
TTCGACG22400.019.9881466
TCAGACC171750.017.8159752
GAACGAT168350.017.3338245
AATACGG169150.017.29207432
ATACGGA168750.017.27973433
GCGTTCT175350.016.770169
TACGGAG171550.016.76161834
TCGGCGC82200.016.2859345
AACGCTA145400.015.86086723
TCGACGA30300.015.7420277
ACGCTAC146800.015.70960424
TTCGGCG84000.015.66909844
CAATACG191400.015.43471331
CGGTATA37650.015.35803320
Produced by FastQC (version 0.11.5)