FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46845685
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG146150.014.67106335
AGCTTAG149850.014.53432845
ATAGGGG157300.014.10325638
GTCACTC212950.013.5346748
CATAGGG166300.013.01533837
GGGAGGT249950.012.9656193
GGTCACT222750.012.7977977
GAGCTTA172050.012.78970444
GCATAGG173150.012.5002936
TGGCATA174300.012.45651334
GGAGGTC248500.012.1984254
AGGTCAC248450.012.0807176
ACTCTCC240200.012.074450511
GAGGTCA254950.012.0205385
TCAGACG78850.010.6746532
TAGGGGA275300.010.62467939
GTTGTCG25750.010.48479526
GCGTAGG68550.010.43727736
TCACTCT283300.010.3645849
CGTAGGG70500.010.24444837
Produced by FastQC (version 0.11.5)