FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49733410
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG720820.1449367738910322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG223700.016.2011945
GGCATAG250500.014.65921635
ATAGGGG255050.014.43026938
GGGAGGT366400.014.39813
GTCACTC347350.014.2676528
GGAGGTC365650.013.9538474
GAGCTTA263500.013.82465744
GGTCACT362150.013.6921987
AGGTCAC372000.013.6803526
TGGCATA270100.013.63706634
GAGGTCA383900.013.3529645
ACTCTCC377600.013.323668511
CATAGGG303550.012.13262137
GTTGTCG20650.012.10177326
TCACTCT412500.012.0960429
GCATAGG319850.011.55719336
CACTCTC428950.011.55322210
TGGGAGG338850.011.4338952
GCTCTGT481250.010.69096122
CTGGGAG415350.010.5014061
Produced by FastQC (version 0.11.5)