FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50395272
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG886380.1758855473584903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG236650.019.0443645
ATAGGGG257000.017.73932638
GGCATAG256550.017.65748835
GAGCTTA266200.017.11589444
GTCACTC347000.016.5561548
GGTCACT357650.016.1132457
TGGCATA282800.016.0745934
CATAGGG284150.015.98187137
GGGAGGT383050.015.8256213
ACTCTCC371250.015.62386511
AGGTCAC383100.015.4100736
GTTGTCG25850.015.14593826
GGAGGTC388650.015.0890374
GAGGTCA392850.015.083475
GCATAGG308300.014.75891436
TCACTCT424650.013.7920269
TGGGAGG347900.013.7253112
CACTCTC438350.013.19833710
TAGGGGA441350.013.00670239
CTGGGAG429400.012.6965251
Produced by FastQC (version 0.11.5)