FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48980743
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG112900.013.45022335
ACCCGGC77350.013.2762055
AGCTTAG118700.012.90642145
ATAGGGG123000.012.41911138
TAGCGTA7900.012.24521237
CATAGGG125000.012.18438537
ACGGACA86200.011.64001218
GCATAGG130900.011.61786336
GAGCTTA142100.011.00262244
CGGACAC95550.010.66640713
CACCCGG101200.010.5925024
TCACCCG107800.010.4456153
TGGCATA151350.010.27109134
ATAGCTC99300.010.26289739
CCGGACA104050.010.11968112
CGGACAG102100.09.98146519
GTCACTC196400.09.9796578
ACTCTCC202750.09.97633911
GCCCGGA110050.09.89554410
GGTCACT195000.09.7859467
Produced by FastQC (version 0.11.5)